Effect of detrusor overactivity on the expression of aquaporins and nitric oxide synthase in rat urinary bladder following bladder outlet obstruction

Authors

  • Sun-Ouck Kim Department of Urology, Chonnam National University Medical School, Gwangju, Korea
  • Dongjune Choi Department of Urology, Chonnam National University Medical School, Gwangju, Korea
  • Seung Hee Song Department of Urology, Chonnam National University Medical School, Gwangju, Korea
  • Kyu Youn Ahn Department of Anatomy, Chonnam National University Medical School, Gwangju, Korea
  • Dongdeuk Kwon Department of Urology, Chonnam National University Medical School, Gwangju, Korea
  • Kwangsung Park Department of Urology, Chonnam National University Medical School, Gwangju, Korea
  • Soo Bang Ryu Department of Urology, Chonnam National University Medical School, Gwangju, Korea

DOI:

https://doi.org/10.5489/cuaj.993

Keywords:

aquaporins, nitric oxide synthase, bladder, obstruction

Abstract

Background: Aquaporins (AQPs) have recently been reported to be expressed in rat and human urothelium. Nitric oxide (NO) is thought to play a role in the bladder overactivity related to bladder outlet obstruction (BOO). The purpose of this study is to investigate the effect of BOO on the expression of AQP2-3 and nitric oxide synthase (NOS) isoforms in rat urothelium.

Methods: Female Sprague-Dawley rats (230-240 g, n = 60) were divided into 2 groups. The control group (n = 30) and the partial bladder outlet obstruction (BOO) group (n = 30). After 4 weeks, we performed a urodynamic study to measure the contraction interval and contraction pressure. The expression and cellular localization of AQP2-3, endothelial nitric oxide synthase (eNOS) and neuronalnitric oxide synthase (nNOS) were determined by Western blot and immunohistochemistry.

Results: On the cystometrogram, the estimated contraction interval time (minutes, mean ± SE) was significantly lower in the BOO group (3.0 ± 0.9) than in the control group (6.3 ± 0.4; p < 0.05). AQP2 was localized in the cytoplasm of the epithelium, whereas AQP3 was found only in the cell membrane of the epithelium. The protein expression of AQP2-3, eNOS and nNOS was significantly increased in the BOO group.

Conclusion: Detrusor overactivity induced by BOO causes a significant increase in the expression of AQP2-3, eNOS, and nNOSin rat urinary bladder. This may imply that the AQPs and NOS isoforms have a functional role in the bladder dysfunction that occurs in association with BOO.

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Published

2013-05-13

How to Cite

Kim, S.-O., Choi, D., Song, S. H., Ahn, K. Y., Kwon, D., Park, K., & Ryu, S. B. (2013). Effect of detrusor overactivity on the expression of aquaporins and nitric oxide synthase in rat urinary bladder following bladder outlet obstruction. Canadian Urological Association Journal, 7(5-6), e268–74. https://doi.org/10.5489/cuaj.993

Issue

Section

Original Research