The effect of astaxanthin on human sperm parameters after cryopreservation
Keywords:Astaxanthin, Cryopreservation, Infertility, Sperm Motility.
Introduction: The aim of the study was to examine the protective efficacy of astaxanthin (ASTA) against the damage that occurs during sperm cryopreservation.
Methods: This experimental study was carried out on waste semen samples of 30 normozoospermic individuals who applied for semen analysis. Semen samples were divided into four equal volumes and 0 μM (control group), 50 μM, 100 μM, and 500 μM ASTA were added to each group. All groups were stored frozen in a liquid nitrogen tank. Semen samples were removed from liquid nitrogen after 72 hours and were thawed. Motility evaluation of sperm was performed. In addition, sperm was stained with acidic aniline blue to detect DNA chromatin condensation.
Results: The highest motility loss was found in the control group and the least motility loss was in the 100 μM ASTA group. When examined in terms of sperm chromatin condensation, condensed sperm count was higher in the 100 μM ASTA group than in the other groups.
Conclusions: It has been observed that ASTA added to the cryoprotectant substance during sperm cryopreservation positively affects sperm motility and reduces the number of decondensed sperm.
How to Cite
You, the Author(s), assign your copyright in and to the Article to the Canadian Urological Association. This means that you may not, without the prior written permission of the CUA:
- Post the Article on any Web site
- Translate or authorize a translation of the Article
- Copy or otherwise reproduce the Article, in any format, beyond what is permitted under Canadian copyright law, or authorize others to do so
- Copy or otherwise reproduce portions of the Article, including tables and figures, beyond what is permitted under Canadian copyright law, or authorize others to do so.
The CUA encourages use for non-commercial educational purposes and will not unreasonably deny any such permission request.
You retain your moral rights in and to the Article. This means that the CUA may not assert its copyright in such a way that would negatively reflect on your reputation or your right to be associated with the Article.
The CUA also requires you to warrant the following:
- That you are the Author(s) and sole owner(s), that the Article is original and unpublished and that you have not previously assigned copyright or granted a licence to any other third party;
- That all individuals who have made a substantive contribution to the article are acknowledged;
- That the Article does not infringe any proprietary right of any third party and that you have received the permissions necessary to include the work of others in the Article; and
- That the Article does not libel or violate the privacy rights of any third party.