Case series – COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis

Authors

  • John M. Masterson Cedars-Sinai Medical Center https://orcid.org/0000-0002-0388-1670
  • Chau Bui Cedars-Sinai Medical Center
  • Wohaib Hasan Cedars-Sinai Medical Center
  • Yi Zhang Cedars-Sinai Medical Center
  • Carissa Hyunh Cedars-Sinai Medical Center
  • Harneet Jawanda Cedars-Sinai Medical Center
  • Daniel Luthringer Cedars-Sinai Medical Center
  • Warren Tourtellotte Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles
  • Eric Vail Cedars-Sinai Medical Center
  • Maurice M. Garcia Cedars-Sinai Medical Center

DOI:

https://doi.org/10.5489/cuaj.7850

Keywords:

COVID-19, male fertility, urology

Abstract

Introduction: We aimed to analyze the testicular histopathology of men who died with active COVID-19 infection.

Methods: We performed autopsy of eight consecutive men who died of COVID-19 pneumonia. Lung and testis tissue of all men were stained for SARS-CoV-2 nucleocapsid, angiotensin-converting enzyme 2 (ACE-2) receptor immunohistochemistry (IHC). H&E was performed to assess for spermatogenesis and evidence of testicle tissue damage. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis for SARS-CoV-2 was performed on matched lung and bilateral testicular tissue samples from all men.

Results: Patient age ranged from 50–79 years. SARS-CoV-2 viral RNA was detected by RTPCR in testis tissue in one man. All eight testicle specimens that underwent IHC for ACE2 receptor showed uniformly strong immunoreactivity against all testicle cell populations. By H&E, all testis specimens showed no inflammation, vascular thrombosis, vasculitis, or morphological evidence of viral changes. One case showed diminished but not absent spermatogenesis, consistent with patient age.

Conclusions: Our results suggest that SARS-CoV-2 is unlikely to affect male fertility. Contrary to all prior histological studies, our results showed no evidence of damage to reproductive tissues that might impair fertility.

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Published

2022-06-09

How to Cite

Masterson, J. M., Bui, C., Hasan, W., Zhang, Y., Hyunh, C., Jawanda, H., Luthringer, D., Tourtellotte, W. ., Vail, E. ., & Garcia, M. M. (2022). Case series – COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis. Canadian Urological Association Journal, 16(11), E558–62. https://doi.org/10.5489/cuaj.7850

Issue

Vol. 16 No. 11 (2022): CUAJ November

Section

Case Series

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