An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Authors

  • Eray Kemahli Abant Izzet Baysal University, School of Medicine
  • Mevlüt Yildiz Düzce Atatürk State Hospital, Düzce,
  • Tülin Firat Abant Izzet Baysal University, School of Medicine, Bolu,
  • Mehmet Emin Ӧzyalvaçlı Abant Izzet Baysal University, School of Medicine
  • Uğur Üyetürk Abant Izzet Baysal University, School of Medicine
  • Burak Yilmaz Abant Izzet Baysal University, School of Medicine
  • Adnan Gücük Abant Izzet Baysal University, School of Medicine

DOI:

https://doi.org/10.5489/cuaj.3160

Abstract

Introduction: The aim of this experimental study was to investigate the histopathological and biochemical effects of pyrrolidine dithiocarbamate, an antioxidant and inhibitor of NF-kβ, on ischemiareperfusion injury in rats.

Methods: A total of 21 male Wistar-Albino rats were randomly distributed into three groups as sham group (Group 1), ischemia-reperfusion (I/R) group (Group 2) and I/R with pyrrolidine dithiocarbamate
(PDTC) group (Group 3). Left testicles of rats in Groups 2 and 3 underwent testicular torsion of 720° for four hours and 100 mg/kg of PDTC was administered intraperitoneally prior to detorsion in Group 3. An hour after detorsion process, left orchiectomies were performed and 5 ml of intracardiac blood samples were drawn from rats in all three groups. Histopathological examination of testis tissues performed and measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in blood samples were taken.

Results: Elevated levels of MDA and decreased SOD activity, together with decreased Johnson tubular biopsy scores consistent with I/R injury were observed in Group 2 (p<0.05). Group 1 and Group 3 were similar in terms of MDA levels, SOD activity, and Johnson scores (p>0.05).

Conclusions: Our results indicated that PDTC may have beneficial effects for alleviation of I/R injury in testicular tissue in rats. Understanding the underlying mechanisms and exploration of its diagnostic and therapeutic potential requires further randomized, controlled trials on a larger scale.

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Author Biographies

Eray Kemahli, Abant Izzet Baysal University, School of Medicine

Department of Urology

Mevlüt Yildiz, Düzce Atatürk State Hospital, Düzce,

Department of Urology

Tülin Firat, Abant Izzet Baysal University, School of Medicine, Bolu,

Department of Histology and Embryology

Mehmet Emin Ӧzyalvaçlı, Abant Izzet Baysal University, School of Medicine

Department of Urology

Uğur Üyetürk, Abant Izzet Baysal University, School of Medicine

Department of Urology

Burak Yilmaz, Abant Izzet Baysal University, School of Medicine

Department of Urology

Adnan Gücük, Abant Izzet Baysal University, School of Medicine

Department of Urology

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Published

2016-03-14

How to Cite

Kemahli, E., Yildiz, M., Firat, T., Ӧzyalvaçlı M. E., Üyetürk, U., Yilmaz, B., & Gücük, A. (2016). An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis. Canadian Urological Association Journal, 10(3-4), E104–9. https://doi.org/10.5489/cuaj.3160

Issue

Vol. 10 No. 3-4 (2016): CUAJ March

Section

Original Research

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