Endogenous biotin expression in renal and testicular tumors and literature review


  • Nader Fahmy
  • Mark Woo
  • Mona Alameldin
  • King Chien Joe Lee
  • Kyle MacDonald
  • Lee W Goneau
  • Peter Cadieux
  • Jeremy Burton
  • Stephen E Pautler




Biotin, renal neoplasms, testicular neoplasms


Introduction: The aim of this study was to examine endogenous biotin levels in tumour specimens collected from patients with renal and testicular tumours and compare them to the surrounding non-neoplastic surgical margin.

Methods: Frozen samples were obtained from the Ontario Tumour Bank. Renal and testicular tumour tissue were included in this study. Normal tissue from the negative surgical margins of each tumour served as a control. Biotin detection in tissue specimens was determined using immunohistochemistry (IHC).

Results: Specimens collected from 56 patients (36 men and 20 women) were included in this study. Histopathology of the 52 renal tumours included 31 (60%) conventional type RCC, 5 (10%) chromophobe RCC, 5 (10%) papillary RCC, 1 (2%) oncocytoma and 10 (19%) upper tract urothelial carcinoma (UC). The 4 testicular tumours included 1 seminomatous (25%) germ cell tumour and 3 (75%) non-seminomatous germ cell tumours.

Conclusion: No biotin signal was perceived in all tested tumour samples. Endogenous biotin expression was detected in the matching non-neoplastic surgical margin of tested renal tissues. This lack of staining may prove to be a valuable tool in future studies.


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How to Cite

Fahmy, N., Woo, M., Alameldin, M., Joe Lee, K. C., MacDonald, K., Goneau, L. W., Cadieux, P., Burton, J., & Pautler, S. E. (2014). Endogenous biotin expression in renal and testicular tumors and literature review. Canadian Urological Association Journal, 8(7-8), 268–72. https://doi.org/10.5489/cuaj.1810



Original Research